The obestatin/ghrelin system as a novel regulatory mechanism of iris muscle contraction.

PURPOSE To evaluate obestatin and ghrelin effects on iris muscle contraction. MATERIALS AND METHODS Obestatin (10(-5) M) or ghrelin (10(-5) M) were tested on two consecutive carbachol-or epinephrine-elicited contractions of iris rabbit sphincter or dilator muscles. Ghrelin and obestatin effects on iris muscles basal tension were also tested, and their effects on iris sphincter EFS-elicited contraction were evaluated. RESULTS Compared with the first, tension of the second carbachol-induced contraction of the iris sphincter decreased 11.5+/-5.5% in the vehicle group, increased 19.0+/-10.2% in presence of obestatin, and remained unchanged by ghrelin. Epinephrine-induced contractions were not affected by obestatin or ghrelin. EFS-elicited contractions were decreased 9.3+/-3.2% by ghrelin. Basal tension of the iris sphincter decreased 21.7+/-3.7% in presence of ghrelin (10(-5) M), while that of the dilator decreased 14.1+/-5.0% in presence of obestatin (10(-5) M). CONCLUSION This study suggests that obestatin potentiates the cholinergic contraction of the iris sphincter and relaxes the iris dilator muscles.